Structure Determination

Comprehensive structural generation solutions for today´s challenging targets provide insights and data to support hit finding through to lead optimization.



XPRESS Service


EGFR, 1.7 Å
EGFR, 1.7 Å

Your target protein or a domain of interest might already be in our XPRESS portfolio. This service provides fast insight into the binding mode of your proprietary compound. To achieve this, CRELUX has optimized expression, purification and crystallization conditions for well behaved XPRESS targets.

Download XPRESS Portfolio

  • Established conditions
  • 150+ disease-relevant targets
  • Average 2 months turn-around



XPEDITE Service


CDK2-Cylin A, 2.0 Å
CDK2-Cylin A, 2.0 Å

Is your target protein of interest not present in our XPRESS list even though there are several published structures available? Then the XPEDITE Service is the option of choice.

Our XPEDITE list primarily consists of more demanding targets, for which we have established in-house protocols for crystallization. In addition, this service grants you quick access to ligand-target protein complex structures, when the crystallization conditions are well described in the literature. XPEDITE service also include the work on disease relevant mutations of established targets and the optimization of published but low resolution structures.

Download XPEDITE Portfolio

  • Established and/or published conditions
  • Few hundred disease-relevant targets
  • Average 4 months turn-around


XPERT Service


MALT1, 2.0 Å
MALT1, 2.0 Å

Customized Structural Biology Solutions

Under XPERT services we offer customized structural biology solutions for your challenging targets, using either crystallography, Cryo-EM or NMR.

Our team of experts will work closely with you to prepare a sound scientific plan with clear milestones to address your needs and lead you to successful de novo structure determination. This blueprint is then translated into a flexible and success-based payment plan, which is accompanied by personalized project management.

  • De novo structure determination
  • Challenging targets (multi-subunit complexes, membrane proteins…)
  • Crystallography, Cryo-EM, NMR
  • Average > 4 months turn-around


X-ray crystallography


Advantages:

  • Broad range of molecular weights
  • Soluble proteins, membrane proteins as well as macromolecular complexes (e.g. DNA/RNA and protein complexes)
  • High resolution (< 3Å)

Disadvantages:

  • The protein of interest must be crystallized and crystals must diffract to the required resolution
  • The 3D structure represents a static form of the target protein

Cryo-EM


Advantages:

  • Allows the structure determination of samples that are unamenable to crystallization (e.g. proteins with flexible regions, membrane proteins and large complexes)
  • Vitrification of the sample maintains it in a closer-to-native state than crystallization
  • Very low material consumption (about 0.1 mg)
  • No need for extensive protein engineering to improve the likelihood of crystallization

Disadvantages:

  • Relatively low resolution (mostly > 3Å) compared to X-ray crystallography
  • Applicable to target proteins and complexes with high molecular weights

NMR


Advantages:

  • 3D structure of target protein can be measured directly in its natural state in solution
  • It can provide unique information about dynamics and intermolecular interactions

Disadvantages:

  • The NMR spectrum of biomolecules with large molecular weight (> 40 kDa) is complicated and difficult to interpret
  • It is necessary to have large amounts of labelled protein (and it might be necessary to have differently labelled protein, if the spectra needs to be assigned)

Ready To Start your Project?

Your discovery projects deserve to receive the best possible support – talk to the experts!

Contact Us