By accessing over 15 years of scientific expertise in protein production, you can be sure that your key target protein meets the desired unique requirements and specifications.
For each family-i.e. kinases, DNA- and RNA- modifying enzymes, membrane proteins, etc.- gain access to our established systems, strategies and valuable experience that address even
the most significant challenges of recombinant protein production.
Download more information (CRELUX PROTEIN)
|Protein Production (Bacteria, insect, mammalian, and yeast cells)|
|Isotope Labeling (15N, 13C)|
|Custom conjugation (e.g., biotinylation)|
|Antigen (protein and peptide) production services|
|Antibody and nanobody production services|
|All target classes|
|over 1200 Assay Grade Proteins (verified for biophysical methods)|
|over 250 Crystallization Grade Proteins (verified crystallization protocols)|
|Increasing collection of Cryo-EM grade proteins|
Our team of experts will closely examine your needs and design the protein constructs tailored to your downstream applications. The construct of your target protein will encompass exactly the sequence you want to examine and will contain the tags required in your assays, ranging from the SPR kinetics measurements to high throughput screenings (HTS). Special attention is dedicated to protein solubility and prevention of aggregation.
For structure generation by crystallography, intensive protein engineering and processing includes the removal of flexible loops, domain exchange, surface entropy reduction mutations etc.
Our innovative approaches
in protein expression have led to generations of stringently controlled high quality
proteins – for in-house use or for the delivery to the client.
You can chose from any of the main expression systems, i.e., bacteria, insect, yeast or mammalian cell lines.
CRELUX PROTEINS are subject to strict quality control measures. The identity of recombinant protein is routinely confirmed by corresponding mass spectrometry analysis. We routinely use analytical size-exclusion chromatography (SEC) to monitor the protein preparation quality, evaluate protein stability, study complex formation, evaluate the tendency of target protein to aggregate and estimate the quantity of aggregates, evaluate the tendency of target protein to degrade and quantify degraded forms. When requested, we can also identify the protein interaction partners and interaction conditions. Typically, our Assay Grade proteins have purity of > 90%. CRELUX Crystal Grade PROTEIN is accompanied by a verified crystallization buffer and crystallization protocol.
Solution NMR is a powerful method for characterizing protein motions over a wide range of time scales by the measurement of relaxation rates of the desired nuclei. Thus, solution NMR spectroscopy often relies on uniform enrichment of 13C and 15N for computational modeling of 3D protein structures.
Depending on the NMR experimental design, we produce proteins containing the desired isotope with desired specificity (e.g., unique residue labeling). These proteins undergo stringent quality control where the efficiency of isotope incorporation is determined by mass spectrometry.
Various assays, such as surface plasmon resonance (SPR), microscale thermophoresis (MST), flow cytometry or immunoassays, require conjugated proteins to assess protein-protein interactions. Importantly, the conjugation of biotin, fluorophore or other chemical entity to the protein may not affect protein structure or activity. Therefore, site-specific conjugations may be recommended. The free conjugates are removed and the conjugation rate determined by given analysis method.
Obtaining a labeled and unlabeled protein from the same source provides a unique advantage in assay development because the label is the only difference between the proteins.
High quality antigen is the key factor to successfully develop antibodies. Factors such as intact protein conformation and appropriate glycosylation pattern significantly influence the development of neutralizing antibodies. Quality control encompasses purity, protein folding, intact mass and mass fingerprint as well as the endotoxin level determination.
In order to support the increasing need of rapid-expression of antibodies, we offer high-throughput antibody expression and purification services. This service combines rapid gene synthesis, vector construction, transient and stable antibody expression. Using our state-of-the-art mammalian cell expression technology, we generate high-purity research-grade antibodies. Our extensive antibody producing experience made assure confidence and ease to achieve customized antibody production solutions in HEK293/CHO/hybridoma cells.